As a means to increase its utility in feed applications, thereby expanding the market of soya bean coproducts, we investigated the simultaneous displacement of marine ingredients in aquafeeds with soya bean‐based protein and a high Omega‐3 fatty acid soya bean oil, enriched with alpha‐linolenic and stearidonic acids, in both steelhead trout ( Oncorhynchus mykiss) and Kampachi ( Seriola rivoliana). Hence, its primary usage is in direct human consumption. The former is the primary vegetable oil consumed in the world. Genetic approaches to add value to either component are ongoing efforts in soya bean breeding and molecular biology programmes. Soya bean ( Glycine max (L.) Merr.) is sought after for both its oil and protein components. Table S11 Fatty acid profiles and carotenoid content of mature soya bean seed from field harvest (2013) Table S10 Fatty acid profiles and carotenoid content of mature soya bean seed from field harvest (2012) Table S9 Fatty acid profiles, total oil and carotenoid content on selected orange colour F 2 seeds producing EPA Table S8 Fatty acid profiles on selected orange colour F 1 seeds producing ALA, SDA or EPA Table S7 Relative percentage of carotenoids in seed of selected transgenic soya bean events
Table S6 Fatty acid profiles on F 3 seeds producing EPA Table S5 Fatty acid profiles on F 1 populations Table S4 Fatty acid profiles on T 1 seed derived from pPTN809D5
Table S3 Fatty acid profile of Kampachi flesh fed diets with increasing percentage of SDA Table S2 Amino acid profiles of aquafeed SPC test formulations Table S1 Aquafeed test formulations with incremental SPC inclusion rates To this end, the systematic introduction of seven transgenic cassettes into soya bean, and the molecular and phenotypic evaluation of the derived novel events are described.įigure S1 T‐DNA elements of pPTN 809D5 and pASTA binary vectors.įigure S2 Southern blot analysis on selected pPTN809D soya bean events.įigure S3 Northern blot analyses on four‐gene stack soya bean lineage derived from SDA event (535‐9) × 824‐1 event (pPTN809D).įigure S4 Phenotypic coloration of soya bean seeds and derived oil from selected pASTA events.įigure S5 Southern blot analyses on selected transgenic soya bean events (pASTA).įigure S6 Northern blot analyses on selected immature T 2 generation embryos obtained from selected pASTA soya bean events.įigure S7 Astaxanthin and β‐carotene levels in selected transgenic soya bean (pASTA) seed grown under greenhouse conditions.įigure S8 Astaxanthin and ß‐carotene levels in selected transgenic soya bean (pASTA) seed grown under field conditions.įigure S9 Tocopherol and tocotrienol levels in selected transgenic soya bean (pASTA) seed grown under greenhouse conditions. Building off of these findings, subsequent efforts were directed towards a genetic strategy that would translate to a prototype design of an optimal identity-preserved soya bean-based feedstock for aquaculture, whereby a multigene stack approach for the targeted synthesis of two value-added output traits, eicosapentaenoic acid and the ketocarotenoid, astaxanthin, were introduced into the crop. Communicated herein are aquafeed formulations with major reduction in marine ingredients that translates to more total Omega-3 fatty acids in harvested flesh. As a means to increase its utility in feed applications, thereby expanding the market of soya bean coproducts, we investigated the simultaneous displacement of marine ingredients in aquafeeds with soya bean-based protein and a high Omega-3 fatty acid soya bean oil, enriched with alpha-linolenic and stearidonic acids, in both steelhead trout (Oncorhynchus mykiss) and Kampachi (Seriola rivoliana). Soya bean (Glycine max (L.) Merr.) is sought after for both its oil and protein components.